Oamenii de știință au imprimat 3D celule testiculare umane

Oamenii de știință din Canada British Columbia (UBC) folosesc tehnologia 3D pentru a imprima celule testiculare umane și au descoperit că acestea au semnele timpurii ale spermatozoizilor pentru a produce spermatozoizi, ceea ce este pentru prima dată în lume. Echipa de cercetare a Dr. Ryan Flannigan, profesor asistent la UBC Urology Assistant, are o-zi, care oferă o soluție pentru pacienții care în prezent nu pot trata.

"15 percent of the couple suffer from infertility, at least half of them is caused by male factors." Flannigan said its laboratory established a Vancouver's prostate center in Vancouver General Hospital. We are printing testicular cells into a special structure that imitates the human anatomy with 3D printing technology, which we believe this is the best way to stimulate sperm generation. "Flannigan said, if success, this will open the new birth treatment door for those who have no other choice.

În testiculele umane, sperma este produsă de un tub mic numit tub fin. În cea mai severă infertilitate masculină-non-obstructivă non-boală a spermatozoizilor (NOA), spermatozoizii se găsesc în ejaculare datorită reducerii spermatozoizilor produse de aceste structuri tubulare mici. Deși, în unele cazuri, medicul poate ajuta pacienții cu NOA să găsească spermatozoizi extrem de rari prin intervenții chirurgicale, dar Flannigan a spus doar jumătate din rata de succes.

"Unfortunately, for the other half of patients, they have no choices, because we can't find sperm for them." Flannigan said, these is the patient who wants to help. In recent studies, researchers conducted biopsy for testis of NOA patients and collected stem cells. These cells were then cultured, and 3D was printed into a hollow tubular structure on the petri dish, similar to the fine elipes capable of producing sperm.

After 12 days, the research team found that these cells did not only survive, but also growing several special cells participating in sperm production, and there was significant improvements in the maintenance of stem cells - this is the early signs of sperm production capacity. The results of this study were recently published in "Plastic and Infertility Science".

"These cells survive and start differentiation, which is a huge milestone. Although there is still a long way to go, this makes our team full of hope." Flannigan said.

The team is currently committed to the "training" cells producing sperm. In order to do this, they exposed cells in different nutrients and growth factors, and fine-tuned their structural arrangements to promote interaction between cells. If these cells can produce sperm, these sperm may pass through in vitro fertilization Make the egg fertilization and provide a new birth treatment option for the couple.

Proiectul de cercetare dezvăluie, de asemenea, mecanismele genetice și moleculare care duc la NOA. Echipa Flannigan a folosit o varietate de tehnici de secvențiere cu un singur-celulă pentru a înțelege expresia genelor și caracteristicile fiecărei celule, apoi a calculat modelul pentru a înțelege mai bine cauza principală a bolii și pentru a determina un nou plan de tratament. Această activitate necesită, de asemenea, o colaborare ridicată, implicând cercetători UBC în informatică, matematică și inginerie și alte cooperări internaționale.

"We are increasingly learned that infertility may have many different reasons, and every case varies from person to person." Flannigan said, "Considering this, we are taking a personalization, precise Medical Methods - Extracting the cells from the patient, trying to understand its unique abnormal conditions, and then supported by 3D printing to overcome these original defects. "